PDF | Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted. PDF | Gerbera jamesonii (gerbera) is an important cut-flower in the global floricultural industry. Micropropagation is the main system used to. PDF | In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L-1 BAP and mg .

Author: Tygole Yozshutilar
Country: Spain
Language: English (Spanish)
Genre: Love
Published (Last): 1 June 2007
Pages: 163
PDF File Size: 11.20 Mb
ePub File Size: 6.30 Mb
ISBN: 649-7-81202-397-2
Downloads: 1285
Price: Free* [*Free Regsitration Required]
Uploader: Shakazil

Micropropagation of Ornamental Plants. Growth Optimization and Organogenesis of Gerbera jamesonii Bolus ex. Plant hormones and type of explants play very important roles in determining regeneration of Gerbera jamesonii in vitro.

Various explants have been used for direct shoot formation. Structural, physiological and biochemical aspects. Petiole segment was identified as the best explant for regeneration of this species. Gerbera jamesonii Bolus ex.

Micropropagation of Gerbera (Gerbera jamesonii Bolus).

In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. The combination of low concentration of cytokinin and auxin initiated propagation of Begonia species Reuter and Bhandari, Shoots and roots of chrysanthemum were developed on a single medium containing 1. Micropropagation selected somaclones Begonia Saintpaulia J.

  HIOKI 3280 PDF

The authors wish to thank the Institute of Biological Sciences and University of Malaya, Kuala Lumpur, Malaysia for providing all facilities and financial support. The lowest callus formation was obtained when root explant was cultured on MS medium supplemented with 3.

Percentage of shoot formation and number of shoots per explants produced on MS medium supplemented with different hormones and concentrations after 8 weeks in culture. Rancillac and Nourrisseau improved the performance of micropropagated strawberry plants by decreasing the cytokinin concentration and limiting the number of subcultures.

De novo shoot organogenesis of pinus eldarica medw.

Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from Gerbera petioles. The study was conducted at laboratory B2.

This callus gave the highest dry weight. How to cite this article: Pakistan Journal of Biological Sciences, Vardja and Vardja reported that, the addition of 10 mg L -1 BAP in culture medium increased the formation of adventitious buds of Gerbera at initiation and multiplication stage.

Gerbera jamesonii gerbera is an important cut-flower in micropropagxtion global floricultural industry. It is reported that a good combination of cytokinin and auxin in the culture medium enhanced good shoot formation and plantlet regeneration from explants, for example, adventitious Gerbera shoots were regenerated from flower buds of greenhouse mifropropagation plants Pierik et al.


The right combination of auxin and cytokinin in the micropropagatiln medium determined the effectiveness of micro propagation of Gerbera shoots. The first step towards de novo regeneration is to establish callus or cell suspension cultures.

However, in this study, at a very high concentration of BAP and other growth regulators, development and growth of shoots were inhibited. Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog MS medium supplemented with 2.

Root formation occurred when explants were cultured on medium with higher auxin concentration and lower cytokinin concentration.


Hook by tissue culture technique. On Tu-er-fang derived from Gerbera plants. Different types of auxin and cytokinin combinations were used in order to obtain complete regeneration of Gerbera in vitro. Capitulum explant promoted callus development. Genotype is one of micropropagtion most influential factors on the response of gerbera in vitro.

The regenerated plants were observed to be normal.