Part, Category. Description, 4-bit Shift Register. Company, ETC. Datasheet, Download datasheet. Quote. Find where to buy. Quote. # Store at. °C. 5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb . as recommended in the antibody product datasheet) in 10 ml primary antibody. Datasheet PDF Download – 4-Bit Shift Register, data sheet.
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Additionally, TET proteins can further oxidize 5-hmC to form 5-formylcytosine 5-fC and 5-carboxylcytosine 5-caCboth of which are excised by thymine-DNA glycosylase TDGeffectively linking cytosine oxidation to the base excision repair pathway and supporting active cytosine demethylation 6,7.
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Cell Signaling Technology 5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb – BIOKÉ
Our website shows product prices without login! The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. TET protein-mediated cytosine hydroxymethylation was initially demonstrated in mouse brain and embryonic stem cells 5, 8. Please refer to this page. Please select a country.
74178 Datasheet PDF
Background Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development 1,2. Therefore webshop accounts that were only used to view prices have been deleted. If so we’d love to hear about it. Have trouble resetting your password? Please change your password to something memorable and secure upon logging in.
The upper panel shows the antibody only binding to the DNA fragment containing 5-fC, while the lower panel shows the membrane stained with methylene blue.
(PDF) 74178 Datasheet download
The modified base itself is stable in vivo and interacts with various readers including MeCP2 11, Please create a new account with your shipping and billing details if you would like to be able to order or view your discount.
Contact us and we will help you. The antibody was titrated against a single-stranded DNA oligo containing either unmodified cytosine or differentially modified cytosine 5-mC, 5-hmC, 5-caC, 5-fC. Furthermore, there is an inverse correlation between levels of 5-hmC and histone H3K9 and H3K27 trimethylation, suggesting a role for 5-hmC in gene activation The same sequence of a base pair DNA fragment was generated by PCR using exclusively unmodified cytosine, 5-methylcytosine 5-mC5-hydroxymethylcytosine 5-hmC5-carboxylcytosine 5-caCor 5-formylcytosine 5-fC.
As shown in the graph, the antibody only binds to the datashheet containing 5-fC. Find your product Go. Login Create new account. Login No need to login to view datashet Application References Have you published research involving the use of our products?
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Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development 1,2. Lower amounts of 5-hmC have been reported in various cancers including myeloid leukemia and melanoma 13, Since then this modification has been discovered in many tissues, with the highest levels found in the brain 9.
By proceeding on this website, you agree to the placement of these cookies. While 5-fC and 5-caC appear to be short-lived intermediate species, there is mounting evidence showing that 5-hmC is a distinct epigenetic mark with various unique functions 10, Have you published research involving the use of our products?
Datasheet PDF –
We are committed datasehet providing you with a productive and privacy-friendly website. The supporting documents available for this product can be downloaded below. Many cells and tissues contain very low endogenous levels of 5-fC that may fall below the detection limits of this antibody. You will receive an e-mail containing a link to start the recovery procedure within minutes.
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