ASTM D – 15 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified. RRIM (MS P) and the ASTM (D ) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRiMt. ASTM De1, Standard Specification for Rubber Surgical Gloves ASTM D, Standard Test Method for the Analysis of Aqueous.

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If you like to setup a quick demo, let us know at support madcad. While donning and removing powdered gloves, the protein-coated particles become air borne dust and can contaminate large volumes.

ASTM D5712 – 15

Potential hazards include skin absorption of harmful substances, chemical or thermal burns, electrical dangers, bruises, abrasions, cuts, punctures, fractures and amputations. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard. Protein levels are below 1, the lowest level of v5712, as clean as a vinyl film negative control.

However, the low silica concentrations necessary make this a cost-effective process for addressing protein allergy concerns.

D Specification for Rubber Surgical Gloves.

Test Results – Vytex

An additional advantage is enhancing latex viscosity resulted only with fumed silica B. However, the 20 ppm proteins left, may happen to be harmful and the latex status as far as allergen content did not change at all. Some of the proteins can be more harmful than others.


The fumed silica attaches itself to the rubber particle and substitutes the proteins. Enter your account email address to request a password reset: Another method of limiting adtm migration is by coating the natural latex surface with a polymer barrier which also provides smoothness. Condoms with different lubricants as typically marketed, have not been tested in an ASTM ILS to determine if, and if so to what degree, the lubricant interferes with the assay.

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Navigation Top Stories Search. The most common mechanism is direct contact. Link to Active This link will always route to the current Active version of the standard. However, traces still may remain and cause skin reactions.

Further analysis by the LEAP method also at Guthrie Research, resulted in clean films having protein values below the detection level, 0. Such powder-free films might have a different kind of a problem. Here again, the presence of silica greatly diminished the level of latex allergenic proteins measured by the LEAP method. Latex protein analysis ASTM D, might not represent a true picture of the latex irritation problem.

PPE-Info – Standard Details

November 20, Content source: Asfm and conclusions Cost and process advantage of latex dipped with fumed silica Presently, latex goods with relatively low protein content are being manufactured by various complicated methods.


Many sstm contain less proteins today, but protein cleaning is an important ongoing issue. It is assumed that all who use this test method will be trained analysts capable of performing common laboratory procedures skillfully and safely. Natural proteins are the main reason for Type I latex irritation.

There is a major distinction between powdered and powder-free gloves. Analytical information demonstrated that powdered latex gloves were found to contain high levels of protein between 1 and micrograms of protein per gram of rubber.

It then could be concluded that the latex has been cleaned. Table III shows results with silica at a lower level of 0. After displacement, the protein become mobile and easier to dispose off.

It is suggested that the silica attaches itself to the surface of the latex particle substituting the protein molecules and displacing them, see Fig. Two-sided coating is possible but expensive.

Natural Rubber Science and Technology, A. Copyright Compu-tecture, Inc.