IDENTIFICATION OF AGAROLYTIC BACTERIA PDF

special ecological reason for the presence of agarolytic bacteria in fresh water but that Identification of Medical Bacteria, 2nd edn. Cam-. Endolytic β-agarase Aga2 was identified from Cellulophaga omnivescoria W5C. SJP92 was shown to retain almost 90% of agarolytic activity under Recently, thermostable agarases from marine bacteria Flammeovirga sp. Abstract: Agarolytic bacteria use agarase to utilize agar as sole source of carbon. It is usually observed in life sciences labs that lot of agar medium needs to be.

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The molecular mass of the enzyme was estimated by gel filtration by using Sephadex G25 and Superdex 75 columns. No activity was observed when other carbon sources, such as glucose or galactose, were used instead of agar as the sole carbon source.

HPLC analysis of the hydrolysis products of unsubstituted agar generated by agarase from P. When enzyme activity was measured in the presence of NaCl in concentrations of up to 0.

Strain N-1 and P. Effect of salt concentration on enzyme activity. National Center for Biotechnology InformationU.

Lane 1, molecular mass standards; lane 2, purified agarase ca. The strain was gram negative, obligately aerobic, and polarly flagellated. Abstract The phenotypic and agarolytic features of an unidentified marine bacteria that was isolated from the southern Pacific coast was investigated.

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Cloning agarolyitc gene replacement mutagenesis of a Pseudomonas atlantica agarase gene. Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. The genera Alteromonas and Marinomonas. Groleau D, Yaphe W.

It requires sodium ion for growth, has an oxidative metabolism, and does not accumulate polyhydroxybutyrate as an intracellular reserve.

At cruder stages the enzyme was strongly bound to DEAE-cellulose, probably through binding to a negatively charged agar or other polysaccharide.

Determination of carbohydrate metabolism of marine bacteria. The activity was determined at a pH between 3.

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Phylogenetic analysis of the genera AlteromonasShewanellaand Moritella using genes coding for small-subunit rRNA sequences of the genus Alteromonas into two genera, Alteromonas emended and Pseudoalteromonas gen.

In our laboratory, we have isolated a few agar-softening and agar-liquefying bacterial strains from the southern Chilean coast to characterize their extracellular agarases in an attempt to contribute to our understanding of the basis of agar hydrolysis.

PMSF was added to a final concentration of 0. In identjfication agar, this isolate produced a diffusible agarase that caused agar softening around the colonies.

To characterize the hydrolysis products of agar with the purified enzyme, a solution of 0. Strain N-1 was ayarolytic in liquid medium containing 0.

Utilization of N -acetylglucosamine, cellobiose, d -fructose, d -galactose, d -glucose, glycogen, inulin, lactose, maltose, d -mannose, mannitol, sacarose, and d -xylose. A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein-dye binding. The release of proteases into the medium during the stationary phase was demonstrated utilizing Azocoll Calbiochem-Behring, La Jolla, Calif.

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Based in these data we propose the assignment of our strain as P. For liquid cultures, agar 0. Sugars were idwntification by filtration through 0. We describe here the identification of a new agarolytic bacterial strain, P. Van der Meulen H, Harder W.

Isolation And Identification of agarolytic bacteria in Marin by Faizah Rahman on Prezi

Enzyme hydrolysis of agar and properties of bacterial agarases. Sequence analysis of the agaB gene encoding a new agarase from Vibrio sp. The enzyme gave a single band on SDS-polyacrylamide aagarolytic Fig.

This effect would be related to the production of low-molecular-weight agarases that can diffuse though the gel pores. K m values of 0. Journal List Appl Environ Microbiol v. The identities of these oligosaccharides were confirmed by thin-layer chromatographic analysis on silica gel plates not shown. The specificity of an agarase from a Cytophaga species. The assays were carried out in 50 mM phosphate pH 7.

Utilization of dl -alanine, l -arginine, l -aspartic acid, creatine, l -cysteine, l -glutamic acid, glycine, l -isoleucine, l agarolytjc, l -ornithine, l -phenylalanine, l -serine, l -threonine, l -tyrosine, and l -valine.